BLV-CoCoMo-qPCR: a useful tool for evaluating bovine leukemia virus infection status
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چکیده
منابع مشابه
BLV-CoCoMo-qPCR: a useful tool for evaluating bovine leukemia virus infection status
BACKGROUND Bovine leukemia virus (BLV) is associated with enzootic bovine leukosis, which is the most common neoplastic disease of cattle. BLV infects cattle worldwide, imposing a severe economic impact on the dairy cattle industry. Recently, we developed a new quantitative real-time polymerase chain reaction (PCR) method using Coordination of Common Motifs (CoCoMo) primers to measure the provi...
متن کاملEstimation of bovine leukemia virus (BLV) proviral load harbored by lymphocyte subpopulations in BLV-infected cattle at the subclinical stage of enzootic bovine leucosis using BLV-CoCoMo-qPCR
BACKGROUND Bovine leukemia virus (BLV) is associated with enzootic bovine leukosis (EBL), which is the most common neoplastic disease of cattle. BLV infection may remain clinically silent at the aleukemic (AL) stage, cause persistent lymphocytosis (PL), or, more rarely, B cell lymphoma. BLV has been identified in B cells, CD2+ T cells, CD3+ T cells, CD4+ T cells, CD8+ T cells, γ/δ T cells, mono...
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The purpose of this study was to determine the prevalence of bovine leukemia virus (BLV) in Khorasan Razavi and Khorasan Shomali provinces which are the main provinces located in the northeast of Iran. Total number of 429 blood samples were collected from industrial dairy herds. The samples were categorized based on province, age (2-3, 4-6, and 7-10 years old), calving (≤ 2, 3-5, and > 5) and h...
متن کاملPurification of a glycoprotein from bovine leukemia virus (BLV).
A precipitating antigen of bovine leukemia virus was isolated by isoelectric focusing and Sephadex gel filtration. In SDS-polyacrylamide gel electrophoresis it was found to be a homogeneous protein with a relative molecular weight of 69000 daltons. Because of its relative molecular weight and staining characteristics it was designated as BLV gp69. A protein with the same molecular weight could ...
متن کاملSeroprevalence of Bovine Leukemia Virus (BLV) Infection in Pregnant Replacement Dairy Heifers in Saraburi Province, Thailand
Seroprevalence of bovine leukemia virus (BLV) infection was studied in replacement dairy heifers. Blood samples were collected from 80 pregnant heifers raised in 8 dairy farms in Saraburi province, the central part of Thailand and serum samples were tested for antibodies against BLV infection using commercially available ELISA test kits. The results revealed that 26 (32.5%) pregnant heifers wer...
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ژورنال
عنوان ژورنال: BMC Veterinary Research
سال: 2012
ISSN: 1746-6148
DOI: 10.1186/1746-6148-8-167